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KMID : 0903619860270030249
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Journal of the Korean Society for Horticultural Science
1986 Volume.27 No. 3 p.249 ~ p.260
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The Influences on the Growth and Controlling Methods of Crown Gall (Agrobacterium tumefaciens SMITH et TOWNSEND) in Dwarf Apple Trees
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김정호/Kim, Jung Ho
김성봉/장한익/임명순/Kim, Seong Bong/Jang, Han Ik/Yiem, Myong Soon
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Abstract
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Studies on the influences and controlling methods of crown gall on the growth and fruiting performance of apple nursery trees in the orchard were carried out using one-year-old apple trees raised by grafting Fuji or Smoothee Golden cultivar on the `M. 26´ rootstock which had been grafted on the apple seedling rootstock one season earlier, and the results obtained from 1978 to 1984 are as follows.
1. No significant results were observed in the treatments testing the healing effects according to the different controlling methods and the different degrees of pre-infection and also in the treatment testing the preventive effects on the apple tree growth and fruit productivity.
2. The deeper soil were the higher soil pH and the exchangeable Ca content within the 15 to 45cm depth, but the retrogradation of crown gall was promoted by deep planting, and air phase of soil was 10.2% in 30 to 45㎝ depth.
3. Ap 8-1 strain among the isolates from the crown galled tissue was identified as a Agrobacterium tumefaciens through the tests of colony shape, color, viscosity on the medium, Bernaerts-De Ley test, Gram staining, shape of bacteria, and inoculation on the bioassay plants.
4. Crown gall was developed vigorously on the stems of castor bean, and tomato received inoculation of Ap 8-1 strain which was isolated from the infected apple trees, and this strain was classified as biovar. 2 type.
5. Lots of meristematic zones and spheroidal bodies in the cell likely to be a lipoid materials which were seen typically in the crown gall tissue were observed in the galled tissue of infected apple tree.
6. It was supposed that the main cause of almost no influences of crown gall on the growth and fruit productivity of apple trees was due to deep planting of double grafted apple nursery trees.
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